Translation and analysis of words by ChatGPT artificial intelligence
On this page you can get a detailed analysis of a word or phrase, produced by the best artificial intelligence technology to date:
- how the word is used
- frequency of use
- it is used more often in oral or written speech
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- etymology
Tag des Kolumbus - translation to English
(aq)23views.png?width=200 "Three views of the Xray structure of Ni(NTA)(H<sub>2</sub>O)<sub>2</sub>")
![a Blossom Twin]].](https://commons.wikimedia.org/wiki/Special:FilePath/Tara tags in Gail Kim.jpg?width=200 "a Blossom Twin]].")
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![[[Lyndon B. Johnson]] in Des Moines on June 30, 1966, near 5th Avenue and the (now-demolished) Hotel Franklin](https://commons.wikimedia.org/wiki/Special:FilePath/A2738-19a small.jpg?width=200 "[[Lyndon B. Johnson]] in Des Moines on June 30, 1966, near 5th Avenue and the (now-demolished) Hotel Franklin")
![Des Moines Municipal Building]]](https://commons.wikimedia.org/wiki/Special:FilePath/Des Moines City Hall.jpg?width=200 "Des Moines Municipal Building]]")
![Astronaut photography of Des Moines taken from the [[International Space Station]]](https://commons.wikimedia.org/wiki/Special:FilePath/Des Moines Iowa.jpg?width=200 "Astronaut photography of Des Moines taken from the [[International Space Station]]")
![Interstate 235]]](https://commons.wikimedia.org/wiki/Special:FilePath/I235 keynote bridge.jpg?width=200 "Interstate 235]]")
![Map of prehistoric and historic American Indian sites in downtown Des Moines<ref>Modified from ''Newsletter of the [[Iowa Archeological Society]]'' 58(1):8</ref>](https://commons.wikimedia.org/wiki/Special:FilePath/Indians of Des Moines.png?width=200 "Map of prehistoric and historic American Indian sites in downtown Des Moines<ref>Modified from ''Newsletter of the [[Iowa Archeological Society]]'' 58(1):8</ref>")
![The [[Iowa State Capitol]], completed in 1886, is one of two state capitols to feature five domes, a central golden dome surrounded by four smaller domes. The other is the [[Rhode Island State House]].](https://commons.wikimedia.org/wiki/Special:FilePath/Iowa State Capitol April 2010.jpg?width=200 "The [[Iowa State Capitol]], completed in 1886, is one of two state capitols to feature five domes, a central golden dome surrounded by four smaller domes. The other is the [[Rhode Island State House]].")
.png?width=200 "Other}}")
![Sec Taylor Field at Principal Park, during a May 28, 2006, game against the [[Nashville Sounds]]. The Iowa Capitol is visible beyond the center-field wall.](https://commons.wikimedia.org/wiki/Special:FilePath/Sec Taylor Field.jpg?width=200 "Sec Taylor Field at Principal Park, during a May 28, 2006, game against the [[Nashville Sounds]]. The Iowa Capitol is visible beyond the center-field wall.")
![The Pappajohn Sculpture Park in the Downtown's [[Western Gateway Park]] plays host to the [[Des Moines Arts Festival]].](https://commons.wikimedia.org/wiki/Special:FilePath/View from the Pappajohn Sculpture Park.jpg?width=200 "The Pappajohn Sculpture Park in the Downtown's [[Western Gateway Park]] plays host to the [[Des Moines Arts Festival]].")
Definition
Wikipedia
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag, by the US trademarked name HIS TAG (US Trademark serial number 74242707), and most commonly as His-Tag. The tag was invented by Roche, although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are available from Qiagen, Sigma, Thermo Scientific, GE Healthcare, Macherey-Nagel, Cube Biotech, Clontech, Bio-Rad, [1] and others.
MK(HQ)6 may be used for enhanced expression in E. coli and tag removal. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. N- or C-terminal His-tags may also be followed or preceded, respectively, by a suitable amino acid sequence that facilitates removal of the polyhistidine-tag using endopeptidases. This extra sequence is not necessary if exopeptidases are used to remove N-terminal His-tags (e.g., Qiagen TAGZyme). Furthermore, exopeptidase cleavage may solve the unspecific cleavage observed when using endoprotease-based tag removal. Polyhistidine-tags are often used for affinity purification of genetically modified proteins.
